• Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit polyclonal antibody (STJ114114) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit polyclonal antibody (STJ114114) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using POFUT2 Rabbit polyclonal antibody (STJ114114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-POFUT2 antibody (240-370) (STJ114114)

SKU:
STJ114114

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POFUT2 (240-370) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POFUT2
Gene ID: 23275
Uniprot ID: OFUT2_HUMAN
Immunogen Region: 240-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).
Immunogen Sequence: MVFARHLREVGDEFRSRHLN STDDADRIPFQEDWMKMKVK LGSALGGPYLGVHLRRKDFI WGHRQDVPSLEGAVRKIRSL MKTHRLDKVFVATDAVRKEY EELKKLLPEMVRFEPTWEEL ELYKDGGVAII
Tissue Specificity Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.
Function Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS, the thrombospondin (TSP) and spondin families (Probable). Required for the proper secretion of ADAMTS family members such as ADAMTSL1 and ADAMTS13. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.
Protein Name Gdp-Fucose Protein O-Fucosyltransferase 2
Peptide-O-Fucosyltransferase 2
O-Fuct-2
Database Links Reactome: R-HSA-5173214
Cellular Localisation Endoplasmic Reticulum
Golgi Apparatus
Mainly Located In The Endoplasmic Reticulum
Alternative Antibody Names Anti-Gdp-Fucose Protein O-Fucosyltransferase 2 antibody
Anti-Peptide-O-Fucosyltransferase 2 antibody
Anti-O-Fuct-2 antibody
Anti-POFUT2 antibody
Anti-C21orf80 antibody
Anti-FUT13 antibody
Anti-KIAA0958 antibody

Information sourced from Uniprot.org

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