• Immunofluorescence analysis of U2OS cells using PNPO Rabbit polyclonal antibody (STJ11100448) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PNPO Rabbit polyclonal antibody (STJ11100448) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PNPO Rabbit polyclonal antibody (STJ11100448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PNPO antibody (1-261) (STJ11100448)

SKU:
STJ11100448

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNPO (1-261) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNPO
Gene ID: 55163
Uniprot ID: PNPO_HUMAN
Immunogen Region: 1-261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PNPO (NP_060599.1).
Immunogen Sequence: MTCWLRGVTATFGRPAEWPG YLSHLCGRSAAMDLGPMRKS YRGDREAFEETHLTSLDPVK QFAAWFEEAVQCPDIGEANA MCLATCTRDGKPSARMLLLK GFGKDGFRFFTNFESRKGKE LDSNPFASLVFYWEPLNRQV RVEGPVKKLPEEEAECYFHS RPKSSQIGAVVSHQSSVIPD REYLRKKNEELEQLYQDQEV PKPKSWGGYVLYPQVMEFWQ GQTNRLHDRIVFRRGLPTG
Tissue Specificity Ubiquitous. Expressed in liver, brain, lung, prostate and stomach (at protein level).
Function Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
Protein Name Pyridoxine-5'-Phosphate Oxidase
Pyridoxamine-Phosphate Oxidase
Database Links Reactome: R-HSA-964975
Alternative Antibody Names Anti-Pyridoxine-5'-Phosphate Oxidase antibody
Anti-Pyridoxamine-Phosphate Oxidase antibody
Anti-PNPO antibody

Information sourced from Uniprot.org

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