• STJ70468 staining (0. 01µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
  • STJ70468 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70468 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Pleckstrin antibody (C-Term) (STJ70468)

SKU:
STJ70468-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Pleckstrin (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.3µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PLEK
Gene ID: 5341
Uniprot ID: PLEK_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002655.2
Immunogen Sequence: EWIKAIQMASRTGK
Protein Name Pleckstrin
Platelet 47 Kda Protein
P47
Database Links Reactome: R-HSA-114608
Alternative Antibody Names Anti-Pleckstrin antibody
Anti-Platelet 47 Kda Protein antibody
Anti-P47 antibody
Anti-PLEK antibody
Anti-P47 antibody

Information sourced from Uniprot.org

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