Western blot analysis of various lysates using PLD4 Rabbit polyclonal antibody (STJ117401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-PLD4 antibody (52-200) (STJ117401)

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STJ117401
€140.63 - €655.63
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLD4
Gene ID: 122618
Uniprot ID: PLD4_HUMAN
Immunogen Region: 52-200
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 52-200 of human PLD4 (NP_620145.2).
Immunogen Sequence: WQVPRPPTWGQVQPKDVPRS WEHGSSPAWEPLEAEARQQR DSCQLVLVESIPQDLPSAAG SPSAQPLGQAWLQLLDTAQE SVHVASYYWSLTGPDIGVND SSSQLGEALLQKLQQLLGRN ISLAVATSSPTLARTSTDLQ VLAARGAHV
Post Translational Modifications Highly N-glycosylated.
Function 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor. Involved in phagocytosis of activated microglia.
Protein Name 5'-3' Exonuclease Pld4
Choline Phosphatase 4
Phosphatidylcholine-Hydrolyzing Phospholipase D4
Phospholipase D Family Member 4
Phospholipase D4
Pld 4
Database Links Reactome: R-HSA-1483148
Reactome: R-HSA-1855204
Reactome: R-HSA-2029485
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Nucleus
Early Endosome
Cytoplasmic Vesicle
Phagosome
Activation Of Microglia Induces Translocation Of Pld4 From The Nucleus To The Phagosomes
Alternative Antibody Names Anti-5'-3' Exonuclease Pld4 antibody
Anti-Choline Phosphatase 4 antibody
Anti-Phosphatidylcholine-Hydrolyzing Phospholipase D4 antibody
Anti-Phospholipase D Family Member 4 antibody
Anti-Phospholipase D4 antibody
Anti-Pld 4 antibody
Anti-PLD4 antibody
Anti-C14orf175 antibody
Anti-UNQ2488 antibody
Anti-PRO5775 antibody

Information sourced from Uniprot.org

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