• Western blot analysis of extracts of various cell lines, using PLA2G2A antibody (STJ11102984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PLA2G2A antibody (65-144) [S4MR] (STJ11102984)

SKU:
STJ11102984

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PLA2G2A (65-144) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLA2G2A
Gene ID: 5320
Uniprot ID: PA2GA_HUMAN
Immunogen Region: 65-144
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 65-144 of human PLA2G2A (P14555).
Immunogen Sequence: VTHDCCYKRLEKRGCGTKFL SYKFSNSGSRITCAKQDSCR SQLCECDKAAATCFARNKTT YNKKYQYYSNKHCRGSTPRC
Tissue Specificity Expressed in various tissues including heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, prostate, ovary, colon and small intestine. Not detected in lymphoid organs and brain. Expressed in platelets (at protein level).
Function Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, promotes the release in circulation of potent damage-associated molecular pattern molecules that activate the innate immune response. Plays a stem cell regulator role in the intestinal crypt. Within intracellular compartment mediates Paneth cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ICS). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates Wnt signaling pathway in ICS cells and tissue regeneration. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.
Protein Name Phospholipase A2 - Membrane Associated
Giic Spla2
Group Iia Phospholipase A2
Non-Pancreatic Secretory Phospholipase A2
Nps-Pla2
Phosphatidylcholine 2-Acylhydrolase 2a
Database Links Reactome: R-HSA-1482788
Reactome: R-HSA-1482801
Reactome: R-HSA-1482839
Reactome: R-HSA-1482922
Reactome: R-HSA-1482925
Reactome: R-HSA-1483166
Reactome: R-HSA-6803157
Cellular Localisation Secreted
Cell Membrane
Peripheral Membrane Protein
Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Phospholipase A2 - Membrane Associated antibody
Anti-Giic Spla2 antibody
Anti-Group Iia Phospholipase A2 antibody
Anti-Non-Pancreatic Secretory Phospholipase A2 antibody
Anti-Nps-Pla2 antibody
Anti-Phosphatidylcholine 2-Acylhydrolase 2a antibody
Anti-PLA2G2A antibody
Anti-PLA2B antibody
Anti-PLA2L antibody
Anti-RASF-A antibody

Information sourced from Uniprot.org

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