| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC/IP/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PKD1/Polycystin 1 (C-Term) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows textured cytoplasmic staining towards the basal membrane in PCT. Recommended concentration: 3-5µg/ml. Paraffin embedded Human Spleen. Recommend |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
PKD1 |
| Gene ID: |
5310 |
| Uniprot ID: |
PKD1_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_001009944.1; NP_000287.2 |
| Immunogen Sequence: |
RTPLRAKNKVHP |
| Function | Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Both PKD1 and PKD2 are required for channel activity. Involved in renal tubulogenesis. Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Acts as a regulator of cilium length, together with PKD2. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria. |
| Protein Name | Polycystin-1Pc1Autosomal Dominant Polycystic Kidney Disease 1 Protein |
| Database Links | Reactome: R-HSA-5620916 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumEndoplasmic ReticulumGolgi ApparatusVesicleSecretedExtracellular ExosomePkd1 Localization To The Plasma And Ciliary Membranes Requires Pkd2Is Independent Of Pkd2 Channel ActivityAnd Involves Stimulation Of Pkd1 Autoproteolytic Cleavage At The Gps Region Of The Gain-B DomainPkd1:Pkd2 Interaction Is Required To Reach The Golgi Apparatus From Endoplasmic Reticulum And Then Traffic To The CiliaCiliary Localization Of Pkd1 Requires Bbs1 And Arl6/Bbs3Cell Surface Localization Requires GanabDetected On Migrasomes And On Extracellular Exosomes In Urine |
| Alternative Antibody Names | Anti-Polycystin-1 antibodyAnti-Pc1 antibodyAnti-Autosomal Dominant Polycystic Kidney Disease 1 Protein antibodyAnti-PKD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
