• Western blot analysis of extracts of various cell lines, using PITRM1 antibody (STJ110660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using PITRM1 Polyclonal Antibody (STJ110660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PITRM1 Polyclonal Antibody (STJ110660) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using PITRM1 Rabbit polyclonal antibody (STJ110660) at 1:6600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PITRM1 antibody (758-1037) (STJ110660)

SKU:
STJ110660

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PITRM1 (758-1037) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PITRM1
Gene ID: 10531
Uniprot ID: PREP_HUMAN
Immunogen Region: 758-1037
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 758-1037 of human PITRM1 (NP_055704.2).
Immunogen Sequence: IKPILRKLPRIKKHLLNGDN MRCSVNATPQQMPQTEKAVE DFLRSIGRSKKERRPVRPHT VEKPVPSSSGGDAHVPHGSQ VIRKLVMEPTFKPWQMKTHF LMPFPVNYVGECIRTVPYTD PDHASLKILARLMTAKFLHT EIREKGGAYGGGAKLSHNGI FTLYSYRDPNTIETLQSFGK AVDWAKSGKFTQQDIDEAKL SVFSTVDAPVAPSDKGMDHF LYGLSDEMKQAHREQLFAV
Tissue Specificity Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
Post Translational Modifications A disulfide bond locks the enzyme in the closed conformation preventing substrate entry into the catalytic chamber.
Function Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently. It is also able to degrade amyloid-beta protein 42.
Protein Name Presequence Protease - Mitochondrial
Hprep
Pitrilysin Metalloproteinase 1
Metalloprotease 1
Hmp1
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Alternative Antibody Names Anti-Presequence Protease - Mitochondrial antibody
Anti-Hprep antibody
Anti-Pitrilysin Metalloproteinase 1 antibody
Anti-Metalloprotease 1 antibody
Anti-Hmp1 antibody
Anti-PITRM1 antibody
Anti-KIAA1104 antibody
Anti-MP1 antibody
Anti-PREP antibody

Information sourced from Uniprot.org

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