| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Pirin (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3-1.0 µg/ml. Primary incubation was 1 hour.IHC: |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PIR |
| Gene ID: | 8544 |
| Uniprot ID: | PIR_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_003653.1 |
| Specificity: | Variants (NP_003653.1; NP_001018119.1) encode the same protein |
| Immunogen Sequence: | GSSKKVTLSVLSREC |
| Function | Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro). |
| Protein Name | PirinProbable Quercetin 2 -3-Dioxygenase PirProbable Quercetinase |
| Database Links | Reactome: R-HSA-8935690 |
| Cellular Localisation | NucleusCytoplasmPredominantly Localized In Dot-Like Subnuclear StructuresCytoplasmic Localization Of Pir Seems To Positively Correlate With Melanoma Progression |
| Alternative Antibody Names | Anti-Pirin antibodyAnti-Probable Quercetin 2 -3-Dioxygenase Pir antibodyAnti-Probable Quercetinase antibodyAnti-PIR antibody |
Information sourced from Uniprot.org