Anti-PICALM antibody (330-380 aa) (STJ13101690)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 330-380 of human PICALM conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-PICALM (330-380 aa) for use in IHC and WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | PICALM |
| Gene ID | 8301 |
| Uniprot ID | PICAL_HUMAN |
| Immunogen | A synthetic peptide from aa region 330-380 of human PICALM conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 330-380 aa |
| Specificity | Specific for PICALM. |
Additional Info
| Function | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF). |
| Protein Name | Phosphatidylinositol-Binding Clathrin Assembly ProteinClathrin Assembly Lymphoid Myeloid Leukemia Protein |
| Database Links | Reactome: R-HSA-432722Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9696264 |
| Cellular Localisation | Cell MembraneMembraneClathrin-Coated PitGolgi ApparatusCytoplasmic VesicleClathrin-Coated VesicleNucleusColocalized With Clathrin In The Golgi AreaInteraction With Pimreg May Target Picalm To The Nucleus In Some Cells |
| Alternative Antibody Names | Anti-Phosphatidylinositol-Binding Clathrin Assembly Protein antibodyAnti-Clathrin Assembly Lymphoid Myeloid Leukemia Protein antibodyAnti-PICALM antibodyAnti-CALM antibody |
Information sourced from Uniprot.org