| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 SUMO-protein ligase PIAS4 (451-500 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIAS4 |
| Gene ID: | 51588 |
| Uniprot ID: | PIAS4_HUMAN |
| Immunogen Region: | 451-500 aa |
| Specificity: | PIASy Polyclonal Antibody detects endogenous levels of PIASy protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PIAS4 at the amino acid range 451-500 |
| Post Translational Modifications | Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin. Mediates sumoylation of KLF8, repressiing KLF8 transcriptional activity and cell cycle progression into G(1) phase. Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity. |
| Protein Name | E3 Sumo-Protein Ligase Pias4 Piasy Protein Inhibitor Of Activated Stat Protein 4 Protein Inhibitor Of Activated Stat Protein Gamma Pias-Gamma |
| Database Links | Reactome: R-HSA-196791 Reactome: R-HSA-3108214 Reactome: R-HSA-3232118 Reactome: R-HSA-3232142 Reactome: R-HSA-3899300 Reactome: R-HSA-4085377 Reactome: R-HSA-4090294 Reactome: R-HSA-4615885 Reactome: R-HSA-4755510 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-69473 |
| Cellular Localisation | Nucleus Pml Body Colocalizes With Sumo1 And Tcf7l2/Tcf4 And Lef1 In A Subset Of Pml (Promyelocytic Leukemia) Nuclear Bodies |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias4 antibody Anti-Piasy antibody Anti-Protein Inhibitor Of Activated Stat Protein 4 antibody Anti-Protein Inhibitor Of Activated Stat Protein Gamma antibody Anti-Pias-Gamma antibody Anti-PIAS4 antibody Anti-PIASG antibody |
Information sourced from Uniprot.org