• Western blot analysis of 293F, using PIAS3 antibody (STJ29140) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of mouse thymus, using PIAS3 antibody (STJ29140) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PIAS3 antibody (STJ29140) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using PIAS3 antibody (STJ29140) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PIAS3 antibody (452-628) (STJ29140)

SKU:
STJ29140

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIAS3 (452-628) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIAS3
Gene ID: 10401
Uniprot ID: PIAS3_HUMAN
Immunogen Region: 452-628
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2).
Immunogen Sequence: IESSSDEEDLPPTKKHCSVT SAAIPALPGSKGVLTSGHQP SSVLRSPAMGTLGGDFLSSL PLHEYPPAFPLGADIQGLDL FSFLQTESQHYGPSVITSLD EQDALGHFFQYRGTPSHFLG PLAPTLGSSHCSATPAPPPG RVSSIVAPGGALREGHGGPL PSGPSLTGCRSDIISLD
Tissue Specificity Widely expressed.
Post Translational Modifications Sumoylated.
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.
Protein Name E3 Sumo-Protein Ligase Pias3
E3 Sumo-Protein Transferase Pias3
Protein Inhibitor Of Activated Stat Protein 3
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-3899300
Reactome: R-HSA-4090294
Reactome: R-HSA-4615885
Reactome: R-HSA-4755510
Reactome: R-HSA-5696395
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Colocalizes With Mitf In The Nucleus
Colocalizes With Gfi1 In Nuclear Dots
Colocalizes With Sumo1 In Nuclear Granules
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Pias3 antibody
Anti-E3 Sumo-Protein Transferase Pias3 antibody
Anti-Protein Inhibitor Of Activated Stat Protein 3 antibody
Anti-PIAS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance