• Western blot analysis of extracts of various cell lines, using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded rat testis tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat testis using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human appendix using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded rat colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using PIAS1+PIAS2 rabbit monoclonal antibody (STJ11102524) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded mouse heart tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded mouse colon tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human liver tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIAS1+PIAS2 in paraffin-embedded human thyroid tissue using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using PIAS1+PIAS2 Rabbit monoclonal antibody (STJ11102524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIAS1/PIAS2 antibody (552-651) [S4MR] (STJ11102524)

SKU:
STJ11102524

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PIAS1+PIAS2 (552-651) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 552-651
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 552-651 of human PIAS1+PIAS2 (O75925).
Immunogen Sequence: DNQHYNTSLLAAAAAAVSDD QDLLHSSRFFPYTSSQMFLD QLSAGGSTSLPTTNGSSSGS NSSLVSSNSLRESHSHTVTN RSSTDTASIFGIIPDIISLD
Background This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Information sourced from Uniprot.org

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