• Immunofluorescence analysis of L929 cells using PI4KA Rabbit polyclonal antibody (STJ11100600) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PI4KA Rabbit polyclonal antibody (STJ11100600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PI4KA antibody (1780-1860) (STJ11100600)

SKU:
STJ11100600

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PI4KA (1780-1860) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PI4KA
Gene ID: 5297
Uniprot ID: PI4KA_HUMAN
Immunogen Region: 1780-1860
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1780-1860 of human PI4KA (P42356).
Immunogen Sequence: PEAIVLDIDYKSGTPMQSAA KAPYLAKFKVKRCGVSELEK EGLRCRSDSEDECSTQEADG QKISWQAAIFKVGDDCRQDM L
Tissue Specificity Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.
Function Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Protein Name Phosphatidylinositol 4-Kinase Alpha
Pi4-Kinase Alpha
Pi4k-Alpha
Ptdins-4-Kinase Alpha
Phosphatidylinositol 4-Kinase Iii Alpha
Database Links Reactome: R-HSA-1483248
Reactome: R-HSA-1660514
Cellular Localisation Cytoplasm
Cell Membrane
Localization To The Plasma Membrane Is Mediated By The Pi4k Complex And Association With Efr3 (Efr3a Or Efr3b)
Ttc7 (Ttc7a Or Ttc7b) And Hycc (Hycc1 Or Hycc2)
Localization To The Plasma Membrane Is Regulated By Tmem150a
Alternative Antibody Names Anti-Phosphatidylinositol 4-Kinase Alpha antibody
Anti-Pi4-Kinase Alpha antibody
Anti-Pi4k-Alpha antibody
Anti-Ptdins-4-Kinase Alpha antibody
Anti-Phosphatidylinositol 4-Kinase Iii Alpha antibody
Anti-PI4KA antibody
Anti-PIK4 antibody
Anti-PIK4CA antibody

Information sourced from Uniprot.org

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