Anti-Phospho-WWOX-Tyr33 antibody (18-67 aa) (STJ90971)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human WWOX around the phosphorylation site of Tyr33 at the amino acid range 18-67
General Information
| Short Description | Rabbit polyclonal anti-Phospho-WW domain-containing oxidoreductase-Tyr33 (18-67 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WWOX |
| Gene ID | 51741 |
| Uniprot ID | WWOX_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human WWOX around the phosphorylation site of Tyr33 at the amino acid range 18-67 |
| Immunogen Region | 18-67 aa |
| Specificity | Phospho-WWOX (Y33) Polyclonal Antibody detects endogenous levels of WWOX protein only when phosphorylated at Y33. |
Additional Info
| Post Translational Modifications | Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation. Ubiquitinated when phosphorylated by TNK2, leading to its degradation. |
| Function | Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. |
| Protein Name | Ww Domain-Containing OxidoreductaseFragile Site Fra16d OxidoreductaseShort Chain Dehydrogenase/Reductase Family 41c Member 1 |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-8866904Reactome: R-HSA-8866907 |
| Cellular Localisation | CytoplasmNucleusMitochondrionGolgi ApparatusLysosomePartially Localizes To The MitochondriaTranslocates To The Nucleus Upon Genotoxic Stress Or Tnf StimulationTranslocates To The Nucleus In Response To Tgfb1Isoform 5 And Isoform 6 May Localize In The NucleusLocalized To The Lysosome Probably Upon Binding To Vopp1 |
| Alternative Antibody Names | Anti-Ww Domain-Containing Oxidoreductase antibodyAnti-Fragile Site Fra16d Oxidoreductase antibodyAnti-Short Chain Dehydrogenase/Reductase Family 41c Member 1 antibodyAnti-WWOX antibodyAnti-FOR antibodyAnti-SDR41C1 antibodyAnti-WOX1 antibody |
Information sourced from Uniprot.org