| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Tensin-2-Tyr483 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TNS2 |
| Gene ID: |
23371 |
| Uniprot ID: |
TENS2_HUMAN |
| Specificity: |
Phospho-Tensin-2 (Y483) Polyclonal Antibody detects endogenous levels of Tensin-2 protein only when phosphorylated at Y483. |
| Immunogen: |
Synthesized phospho-peptide around the phosphorylation site of human Tensin-2 (phospho Tyr483) |
| Post Translational Modifications | Ubiquitinated following sequestration in cytoplasmic aggregates with SQSTM1, leading to proteasomal degradation. |
| Function | Tyrosine-protein phosphatase which regulates cell motility, proliferation and muscle-response to insulin. Phosphatase activity is mediated by binding to phosphatidylinositol-3,4,5-triphosphate (PtdIns(3,4,5)P3) via the SH2 domain. In muscles and under catabolic conditions, dephosphorylates IRS1 leading to its degradation and muscle atrophy. Negatively regulates PI3K-AKT pathway activation. Dephosphorylates nephrin NPHS1 in podocytes which regulates activity of the mTORC1 complex. Under normal glucose conditions, NPHS1 outcompetes IRS1 for binding to phosphatidylinositol 3-kinase (PI3K) which balances mTORC1 activity but high glucose conditions lead to up-regulation of TNS2, increased NPHS1 dephosphorylation and activation of mTORC1, contributing to podocyte hypertrophy and proteinuria. Required for correct podocyte morphology, podocyte-glomerular basement membrane interaction and integrity of the glomerular filtration barrier. Enhances RHOA activation in the presence of DLC1. Plays a role in promoting DLC1-dependent remodeling of the extracellular matrix. |
| Protein Name | Tensin-2C1 Domain-Containing Phosphatase And Tensin HomologC1-TenTensin-Like C1 Domain-Containing Phosphatase |
| Cellular Localisation | Cell JunctionFocal AdhesionCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmDetected At The End Of Actin Stress FibersDetected In Cytoplasmic Punctate BodiesLocalizes To Both Focal Adhesions And Fibrillar Adhesions But Is Found Mainly In Focal AdhesionsEnriched In Dynamic Focal Adhesions At The Leading Edge Of The Cell And Is Found Only Rarely In Fibrillar Adhesions On The Ventral Surface Of Cells |
| Alternative Antibody Names | Anti-Tensin-2 antibodyAnti-C1 Domain-Containing Phosphatase And Tensin Homolog antibodyAnti-C1-Ten antibodyAnti-Tensin-Like C1 Domain-Containing Phosphatase antibodyAnti-TNS2 antibodyAnti-KIAA1075 antibodyAnti-TENC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
