• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Stathmin 1-S38 rabbit polyclonal antibody (STJ22422) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Stathmin 1-S38 Rabbit polyclonal antibody (STJ22422) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-STMN1-S38 antibody (STJ22422)

SKU:
STJ22422

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-STMN1-S38 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STMN1
Gene ID: 3925
Uniprot ID: STMN1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S38 of human Stathmin 1 (NP_001138926.1).
Immunogen Sequence: PLSPP
Tissue Specificity Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Post Translational Modifications Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Function Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Protein Name Stathmin
Leukemia-Associated Phosphoprotein P18
Metablastin
Oncoprotein 18
Op18
Phosphoprotein P19
Pp19
Prosolin
Protein Pr22
Pp17
Database Links Reactome: R-HSA-1251985
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Stathmin antibody
Anti-Leukemia-Associated Phosphoprotein P18 antibody
Anti-Metablastin antibody
Anti-Oncoprotein 18 antibody
Anti-Op18 antibody
Anti-Phosphoprotein P19 antibody
Anti-Pp19 antibody
Anti-Prosolin antibody
Anti-Protein Pr22 antibody
Anti-Pp17 antibody
Anti-STMN1 antibody
Anti-C1orf215 antibody
Anti-LAP18 antibody
Anti-OP18 antibody

Information sourced from Uniprot.org

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