| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-DNA-directed RNA polymerase II subunit RPB1-Ser1619 (1585-1634 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLR2A |
| Gene ID: | 5430 |
| Uniprot ID: | RPB1_HUMAN |
| Immunogen Region: | 1585-1634 aa |
| Specificity: | Phospho-Rpb1 (S1619) Polyclonal Antibody detects endogenous levels of Rpb1 protein only when phosphorylated at S1619. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human POLR2A around the phosphorylation site of Ser1619 at the amino acid range 1585-1634 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines. Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression. (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. |
| Protein Name | Dna-Directed Rna Polymerase Ii Subunit Rpb1Rna Polymerase Ii Subunit B1Dna-Directed Rna Polymerase Ii Subunit ADna-Directed Rna Polymerase Iii Largest SubunitRna-Directed Rna Polymerase Ii Subunit Rpb1 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-168325Reactome: R-HSA-203927Reactome: R-HSA-5578749Reactome: R-HSA-5601884Reactome: R-HSA-5617472Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6803529Reactome: R-HSA-6807505Reactome: R-HSA-72086Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72203Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075Reactome: R-HSA-8851708Reactome: R-HSA-9018519Reactome: R-HSA-9670095 |
| Cellular Localisation | NucleusCytoplasmChromosomeHypophosphorylated Form Is Mainly Found In The CytoplasmWhile The Hyperphosphorylated And Active Form Is NuclearCo-Localizes With Kinase Srpk2 And Helicase Ddx23 At Chromatin Loci Where Unscheduled R-Loops Form |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb1 antibodyAnti-Rna Polymerase Ii Subunit B1 antibodyAnti-Dna-Directed Rna Polymerase Ii Subunit A antibodyAnti-Dna-Directed Rna Polymerase Iii Largest Subunit antibodyAnti-Rna-Directed Rna Polymerase Ii Subunit Rpb1 antibodyAnti-POLR2A antibodyAnti-POLR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
