Anti-Phospho-RPA2-Thr21 antibody (10-59 aa) (STJ90903)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RFA2 around the phosphorylation site of Thr21 at the amino acid range 10-59
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Replication protein A 32 kDa subunit-Thr21 (10-59 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPA2 |
| Gene ID | 6118 |
| Uniprot ID | RFA2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RFA2 around the phosphorylation site of Thr21 at the amino acid range 10-59 |
| Immunogen Region | 10-59 aa |
| Specificity | Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21. |
Additional Info
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. |
| Protein Name | Replication Protein A 32 Kda SubunitRp-A P32Replication Factor A Protein 2Rf-A Protein 2Replication Protein A 34 Kda SubunitRp-A P34 |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-174437Reactome: R-HSA-176187Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-5696395Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6783310Reactome: R-HSA-6804756Reactome: R-HSA-68962Reactome: R-HSA-69166Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusPml BodyRedistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner |
| Alternative Antibody Names | Anti-Replication Protein A 32 Kda Subunit antibodyAnti-Rp-A P32 antibodyAnti-Replication Factor A Protein 2 antibodyAnti-Rf-A Protein 2 antibodyAnti-Replication Protein A 34 Kda Subunit antibodyAnti-Rp-A P34 antibodyAnti-RPA2 antibodyAnti-REPA2 antibodyAnti-RPA32 antibodyAnti-RPA34 antibody |
Information sourced from Uniprot.org