| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Receptor-interacting serine/threonine-protein kinase 1-Ser166 is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RIPK1 |
| Gene ID: |
8737 |
| Uniprot ID: |
RIPK1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human RIP (phospho-Ser166) |
| Immunogen: |
Synthesized phosho peptide around human RIP (Ser166) |
| Function | Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis. In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1. Inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis. Required to inhibit apoptosis and necroptosis during embryonic development: acts by preventing the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8. In addition to apoptosis and necroptosis, also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6). Phosphorylates RIPK3: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Required for ZBP1-induced NF-kappa-B activation in response to DNA damage. |
| Protein Name | Receptor-Interacting Serine/Threonine-Protein Kinase 1Cell Death Protein RipReceptor-Interacting Protein 1Rip-1 |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-168927Reactome: R-HSA-1810476Reactome: R-HSA-2562578Reactome: R-HSA-3295583Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5675482Reactome: R-HSA-5689880Reactome: R-HSA-5689896Reactome: R-HSA-69416Reactome: R-HSA-75893Reactome: R-HSA-9013957Reactome: R-HSA-933543Reactome: R-HSA-937041Reactome: R-HSA-9679191Reactome: R-HSA-9686347Reactome: R-HSA-9692913Reactome: R-HSA-9693928 |
| Cellular Localisation | CytoplasmCell Membrane |
| Alternative Antibody Names | Anti-Receptor-Interacting Serine/Threonine-Protein Kinase 1 antibodyAnti-Cell Death Protein Rip antibodyAnti-Receptor-Interacting Protein 1 antibodyAnti-Rip-1 antibodyAnti-RIPK1 antibodyAnti-RIP antibodyAnti-RIP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
