• Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PLCG1-Tyr1253 antibody (1221-1270 aa) (STJ90475)

SKU:
STJ90475

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1-Tyr1253 (1221-1270 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCG1
Gene ID: 5335
Uniprot ID: PLCG1_HUMAN
Immunogen Region: 1221-1270 aa
Specificity: Phospho-PLC Gamma 1 (Y1253) Polyclonal Antibody detects endogenous levels of PLC Gamma 1 protein only when phosphorylated at Y1253.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PLCG1 around the phosphorylation site of Tyr1253 at the amino acid range 1221-1270
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1
Plc-148
Phosphoinositide Phospholipase C-Gamma-1
Phospholipase C-Ii
Plc-Ii
Phospholipase C-Gamma-1
Plc-Gamma-1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-1236382
Reactome: R-HSA-1251932
Reactome: R-HSA-1489509
Reactome: R-HSA-167021
Reactome: R-HSA-1855204
Reactome: R-HSA-186763
Reactome: R-HSA-201556
Reactome: R-HSA-202433
Reactome: R-HSA-2029485
Reactome: R-HSA-210990
Reactome: R-HSA-212718
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-418890
Reactome: R-HSA-5218921
Reactome: R-HSA-5637810
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-8853659
Reactome: R-HSA-9026527
Reactome: R-HSA-9027277
Reactome: R-HSA-9034793
Reactome: R-HSA-9664323
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9725370
Cellular Localisation Cell Projection
Lamellipodium
Ruffle
Rapidly Redistributed To Ruffles And Lamellipodia Structures In Response To Epidermal Growth Factor (Egf) Treatment
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1 antibody
Anti-Plc-148 antibody
Anti-Phosphoinositide Phospholipase C-Gamma-1 antibody
Anti-Phospholipase C-Ii antibody
Anti-Plc-Ii antibody
Anti-Phospholipase C-Gamma-1 antibody
Anti-Plc-Gamma-1 antibody
Anti-PLCG1 antibody
Anti-PLC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance