Anti-Phospho-PAK6-Ser-165 antibody (STJA0041338)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-PAK6-Ser-165 for use in ELISA and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | PAK6 |
| Gene ID | 106821730/56924 |
| Uniprot ID | PAK6_HUMAN |
| Immunogen | PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6. |
| Immunogen Sequence | Human |
| Specificity | This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa* band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with |
Additional Info
| Tissue Specificity | Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast. |
| Post Translational Modifications | Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation. |
| Function | Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD. |
| Protein Name | Serine/Threonine-Protein Kinase Pak 6Pak-5P21-Activated Kinase 6Pak-6 |
| Database Links | Reactome: R-HSA-428540Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013405Reactome: R-HSA-9013407Reactome: R-HSA-9013424 |
| Cellular Localisation | CytoplasmNucleusCotranslocates Into Nucleus With Ar In Response To Androgen Induction |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Pak 6 antibodyAnti-Pak-5 antibodyAnti-P21-Activated Kinase 6 antibodyAnti-Pak-6 antibodyAnti-PAK6 antibodyAnti-PAK5 antibody |
Information sourced from Uniprot.org