• Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of K562 lysis using Phospho-p38 (T180/Y182) antibody. Antibody was diluted at 1:1000
  • Immunofluorescence analysis of mouse-liver tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using Phospho-p38 (T180/Y182) Polyclonal Antibody diluted at 1:1000
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)
  • Immunofluorescence analysis of mouse-liver tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of HEPG2 cells using Phospho-p38 (T180/Y182) Polyclonal Antibody diluted at 1:1000
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from A549 cells treated with etoposide 25uM 24hours, using p38 MAPK (Phospho-Thr179+Tyr181) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-p38 (T180/Y182) antibody (STJ90498)

SKU:
STJ90498
Current Stock:
Host: Rabbit
Applications: IF, WB, IHC-P, ELISA
Reactivity: Human,Mouse,Rat,Guinea Pig
Short Description: Rabbit polyclonal primary antibody against Phospho-p38 (T180/Y182).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Specificity: Phospho-p38 (T180/Y182) polyclonal antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182.
Uniprot ID: MK14_HUMAN
Gene ID: 1432
Gene Symbol: MAPK14
Immunogen: Synthesized peptide derived from human p38 around the phosphorylation site of T180/Y182.
Immunogen Region: 120-200 aa
Tissue Specificity Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Post Translational Modifications Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14. Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3. Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Database Links Reactome: R-HSA-168638
Cellular Localisation Cytoplasm
Nucleus
Protein Name Mitogen-Activated Protein Kinase 14
Map Kinase 14
Mapk 14
Cytokine Suppressive Anti-Inflammatory Drug-Binding Protein
Csaid-Binding Protein
Csbp
Map Kinase Mxi2
Max-Interacting Protein 2
Mitogen-Activated Protein Kinase P38 Alpha
Map Kinase P38 Alpha
Stress-Activated Protein Kinase 2a
Sapk2a
Alternative Names Anti-Mitogen-Activated Protein Kinase 14 antibody
Anti-Map Kinase 14 antibody
Anti-Mapk 14 antibody
Anti-Cytokine Suppressive Anti-Inflammatory Drug-Binding Protein antibody
Anti-Csaid-Binding Protein antibody
Anti-Csbp antibody
Anti-Map Kinase Mxi2 antibody
Anti-Max-Interacting Protein 2 antibody
Anti-Mitogen-Activated Protein Kinase P38 Alpha antibody
Anti-Map Kinase P38 Alpha antibody
Anti-Stress-Activated Protein Kinase 2a antibody
Anti-Sapk2a antibody
Anti-MAPK14 antibody
Anti-CSBP antibody
Anti-CSBP1 antibody
Anti-CSBP2 antibody
Anti-CSPB1 antibody
Anti-MXI2 antibody
Anti-SAPK2A antibody

Information sourced from uniprot.org

6 months standard