• Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using POU2F1/OCT1 (Phospho-Ser385) Antibody
  • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-Oct-1-Ser385 antibody (361-410 aa) (STJ91181)

SKU:
STJ91181

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-POU domain, class 2, transcription factor 1 and POU domain, class 2, transcription factor 2-Ser385 (361-410 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2F1
POU2F2
Gene ID: 5451
5452
Uniprot ID: PO2F1_HUMAN
PO2F2_HUMAN
Immunogen Region: 361-410 aa
Specificity: Phospho-Oct-1 (S385) Polyclonal Antibody detects endogenous levels of 41183 protein only when phosphorylated at S385.
Immunogen: The antiserum was produced against synthesized peptide derived from the human POU2F1/OCT1 around the phosphorylation site of Ser385 at the amino acid range 361-410
Post Translational Modifications Phosphorylated by PRKDC.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Protein Name Pou Domain - Class 2 - Transcription Factor 1
Nf-A1
Octamer-Binding Protein 1
Oct-1
Octamer-Binding Transcription Factor 1
Otf-1
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6807505
Reactome: R-HSA-749476
Reactome: R-HSA-76071
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 2 - Transcription Factor 1 antibody
Anti-Nf-A1 antibody
Anti-Octamer-Binding Protein 1 antibody
Anti-Oct-1 antibody
Anti-Octamer-Binding Transcription Factor 1 antibody
Anti-Otf-1 antibody
Anti-POU2F1 antibody
Anti-OCT1 antibody
Anti-OTF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance