• Western blot analysis of lysates from NIH/3T3 cells, using Trk B (Phospho-Tyr515) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of RAT-MUSCLE cells using Phospho-Trk B (Y516) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-NTRK2-Tyr516 antibody (481-530 aa) (STJ90429)

SKU:
STJ90429

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-BDNF/NT-3 growth factors receptor-Tyr516 (481-530 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NTRK2
Gene ID: 4915
Uniprot ID: NTRK2_HUMAN
Immunogen Region: 481-530 aa
Specificity: Phospho-Trk B (Y516) Polyclonal Antibody detects endogenous levels of Trk B protein only when phosphorylated at Y516.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Trk B around the phosphorylation site of Tyr515 at the amino acid range 481-530
Post Translational Modifications Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated. Ubiquitinated. Undergoes polyubiquitination upon activation.regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
Function Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
Protein Name Bdnf/Nt-3 Growth Factors Receptor
Gp145-Trkb
Trk-B
Neurotrophic Tyrosine Kinase Receptor Type 2
Trkb Tyrosine Kinase
Tropomyosin-Related Kinase B
Database Links Reactome: R-HSA-187024
Reactome: R-HSA-9024909
Reactome: R-HSA-9025046
Reactome: R-HSA-9026357
Reactome: R-HSA-9026519
Reactome: R-HSA-9026527
Reactome: R-HSA-9028335
Reactome: R-HSA-9028731
Reactome: R-HSA-9032500
Reactome: R-HSA-9032759
Reactome: R-HSA-9032845
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Early Endosome Membrane
Cell Projection
Axon
Dendrite
Cytoplasm
Perinuclear Region
Postsynaptic Density
Internalized To Endosomes Upon Ligand-Binding
Alternative Antibody Names Anti-Bdnf/Nt-3 Growth Factors Receptor antibody
Anti-Gp145-Trkb antibody
Anti-Trk-B antibody
Anti-Neurotrophic Tyrosine Kinase Receptor Type 2 antibody
Anti-Trkb Tyrosine Kinase antibody
Anti-Tropomyosin-Related Kinase B antibody
Anti-NTRK2 antibody
Anti-TRKB antibody

Information sourced from Uniprot.org

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