• Immunohistochemistry analysis of paraffin-embedded human brain, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from A549 cells, using n-NOS (Phospho-Ser852) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS1-Ser852 antibody (818-867 aa) (STJ90355)

SKU:
STJ90355

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nitric oxide synthase 1-Ser852 (818-867 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOS1
Gene ID: 4842
Uniprot ID: NOS1_HUMAN
Immunogen Region: 818-867 aa
Specificity: Phospho-NOS1 (S852) Polyclonal Antibody detects endogenous levels of NOS1 protein only when phosphorylated at S852.
Immunogen: The antiserum was produced against synthesized peptide derived from the human n-NOS around the phosphorylation site of Ser852 at the amino acid range 818-867
Post Translational Modifications Ubiquitinated.mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Protein Name Nitric Oxide Synthase 1
Constitutive Nos
Nc-Nos
Nos Type I
Neuronal Nos
N-Nos
Nnos
Nitric Oxide Synthase - Brain
Bnos
Peptidyl-Cysteine S-Nitrosylase Nos1
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-392154
Reactome: R-HSA-5578775
Cellular Localisation Cell Membrane
Sarcolemma
Peripheral Membrane Protein
Cell Projection
Dendritic Spine
In Skeletal Muscle
It Is Localized Beneath The Sarcolemma Of Fast-Twitch Muscle Fiber By Associating With The Dystrophin Glycoprotein Complex
In Neurons
Enriched In Dendritic Spines
Alternative Antibody Names Anti-Nitric Oxide Synthase 1 antibody
Anti-Constitutive Nos antibody
Anti-Nc-Nos antibody
Anti-Nos Type I antibody
Anti-Neuronal Nos antibody
Anti-N-Nos antibody
Anti-Nnos antibody
Anti-Nitric Oxide Synthase - Brain antibody
Anti-Bnos antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Nos1 antibody
Anti-NOS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance