• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody

Anti-Phospho-ERBB2-Tyr1221/Y1222 antibody (1191-1240 aa) (STJ90337)

SKU:
STJ90337

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Receptor tyrosine-protein kinase erbB-2-Tyr1221/Y1222 (1191-1240 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERBB2
Gene ID: 2064
Uniprot ID: ERBB2_HUMAN
Immunogen Region: 1191-1240 aa
Specificity: Phospho-Neu (Y1221/Y1222) Polyclonal Antibody detects endogenous levels of Neu protein only when phosphorylated at Y1221/Y1222.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HER2 around the phosphorylation site of Tyr1221/Tyr1222 at the amino acid range 1191-1240
Post Translational Modifications Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.
Function Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Protein Name Receptor Tyrosine-Protein Kinase Erbb-2
Metastatic Lymph Node Gene 19 Protein
Mln 19
Proto-Oncogene Neu
Proto-Oncogene C-Erbb-2
Tyrosine Kinase-Type Cell Surface Receptor Her2
P185erbb2
Cd Antigen Cd340
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1250196
Reactome: R-HSA-1251932
Reactome: R-HSA-1257604
Reactome: R-HSA-1306955
Reactome: R-HSA-1358803
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-2219530
Reactome: R-HSA-416572
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8863795
Reactome: R-HSA-8866910
Reactome: R-HSA-9634285
Reactome: R-HSA-9652282
Reactome: R-HSA-9664565
Reactome: R-HSA-9665233
Reactome: R-HSA-9665244
Reactome: R-HSA-9665245
Reactome: R-HSA-9665246
Reactome: R-HSA-9665247
Reactome: R-HSA-9665249
Reactome: R-HSA-9665250
Reactome: R-HSA-9665251
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9665737
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Ruffle Membrane
Internalized From The Cell Membrane In Response To Egf Stimulation
Isoform 1: Cell Membrane
Early Endosome
Cytoplasm
Perinuclear Region
Nucleus
Translocation To The Nucleus Requires Endocytosis
Probably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1
Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde Vesicles
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-Receptor Tyrosine-Protein Kinase Erbb-2 antibody
Anti-Metastatic Lymph Node Gene 19 Protein antibody
Anti-Mln 19 antibody
Anti-Proto-Oncogene Neu antibody
Anti-Proto-Oncogene C-Erbb-2 antibody
Anti-Tyrosine Kinase-Type Cell Surface Receptor Her2 antibody
Anti-P185erbb2 antibody
Anti-Cd Antigen Cd340 antibody
Anti-ERBB2 antibody
Anti-HER2 antibody
Anti-MLN19 antibody
Anti-NEU antibody
Anti-NGL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance