| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 7-Thr275 (241-290 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP2K7 |
| Gene ID: | 5609 |
| Uniprot ID: | MP2K7_HUMAN |
| Immunogen Region: | 241-290 aa |
| Specificity: | Phospho-MEK-7 (T275) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at T275. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human MAP2K7 around the phosphorylation site of Thr275 at the amino acid range 241-290 |
| Post Translational Modifications | Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 Map Kinase Kinase 7 Mapkk 7 Jnk-Activating Kinase 2 Mapk/Erk Kinase 7 Mek 7 Stress-Activated Protein Kinase Kinase 4 Sapk Kinase 4 Sapkk-4 Sapkk4 C-Jun N-Terminal Kinase Kinase 2 Jnk Kinase 2 Jnkk 2 |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-2871796 Reactome: R-HSA-450321 Reactome: R-HSA-5210891 |
| Cellular Localisation | Nucleus Cytoplasm |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 antibody Anti-Map Kinase Kinase 7 antibody Anti-Mapkk 7 antibody Anti-Jnk-Activating Kinase 2 antibody Anti-Mapk/Erk Kinase 7 antibody Anti-Mek 7 antibody Anti-Stress-Activated Protein Kinase Kinase 4 antibody Anti-Sapk Kinase 4 antibody Anti-Sapkk-4 antibody Anti-Sapkk4 antibody Anti-C-Jun N-Terminal Kinase Kinase 2 antibody Anti-Jnk Kinase 2 antibody Anti-Jnkk 2 antibody Anti-MAP2K7 antibody Anti-JNKK2 antibody Anti-MEK7 antibody Anti-MKK7 antibody Anti-PRKMK7 antibody Anti-SKK4 antibody |
Information sourced from Uniprot.org