• Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-MAP2K5-Ser311/Thr315 antibody (STJA0003686)

SKU:
STJA0003686-100

€568.13
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Host: Rabbit
Applications: WB
Reactivity: Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MEK5-Ser311/Thr315 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Map2k5
Gene ID: 29568
Uniprot ID: MP2K5_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.
Tissue Specificity Isoform Alpha-1: Expressed in the liver and brain (at protein level). Isoform Alpha-2: Expressed in the liver and brain (at protein level). Isoform Beta: Expressed in the liver, muscle, testes, lung, kidney, spleen, heart and brain (at protein level).
Post Translational Modifications Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 5
Map Kinase Kinase 5
Mapkk 5
Mapk/Erk Kinase 5
Mek 5
Cellular Localisation Isoform Beta: Cytoplasm
Cytosol
Isoform Alpha-1: Membrane
The Alternatively Spliced Exon In Alpha Isoform Resemble Conserved Sequences That Mediate Interactions With The Cytoskeleton
Thereby Explaining The Differential Localization
Isoform Alpha-2: Membrane
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 5 antibody
Anti-Map Kinase Kinase 5 antibody
Anti-Mapkk 5 antibody
Anti-Mapk/Erk Kinase 5 antibody
Anti-Mek 5 antibody
Anti-Map2k5 antibody
Anti-Mek5 antibody
Anti-Mkk5 antibody
Anti-Prkmk5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance