• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from K562 cells, using HSL (Phospho-Ser552) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-HSL (S552) Polyclonal Antibody diluted at 1：500

Anti-Phospho-LIPE-Ser552 antibody (518-567 aa) (STJ90796)

SKU:
STJ90796

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Hormone-sensitive lipase-Ser552 (518-567 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIPE
Gene ID: 3991
Uniprot ID: LIPS_HUMAN
Immunogen Region: 518-567 aa
Specificity: Phospho-HSL (S552) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S552.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HSL around the phosphorylation site of Ser552 at the amino acid range 518-567
Post Translational Modifications Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Function Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Protein Name Hormone-Sensitive Lipase
Hsl
Monoacylglycerol Lipase Lipe
Retinyl Ester Hydrolase
Reh
Database Links Reactome: R-HSA-163560
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytosol
Lipid Droplet
Found In The High-Density Caveolae
Translocates To The Cytoplasm From The Caveolae Upon Insulin Stimulation
Phosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets
Alternative Antibody Names Anti-Hormone-Sensitive Lipase antibody
Anti-Hsl antibody
Anti-Monoacylglycerol Lipase Lipe antibody
Anti-Retinyl Ester Hydrolase antibody
Anti-Reh antibody
Anti-LIPE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance