• Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-LCP2-Ser376 antibody (STJ196426)

SKU:
STJ196426

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Lymphocyte cytosolic protein 2-Ser376 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCP2
Gene ID: 3937
Uniprot ID: LCP2_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse SLP-76 (phospho-Ser376)
Immunogen: Synthesized phosho peptide around human SLP-76 (Ser376)
Post Translational Modifications Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Function Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK. In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Plays also an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses.
Protein Name Lymphocyte Cytosolic Protein 2
Sh2 Domain-Containing Leukocyte Protein Of 76 Kda
Slp-76 Tyrosine Phosphoprotein
Slp76
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-202433
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Lymphocyte Cytosolic Protein 2 antibody
Anti-Sh2 Domain-Containing Leukocyte Protein Of 76 Kda antibody
Anti-Slp-76 Tyrosine Phosphoprotein antibody
Anti-Slp76 antibody
Anti-LCP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance