Anti-Phospho-LATS1-Ser909 antibody (STJ196345)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human LATS1/2 (Ser909)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Serine/threonine-protein kinase LATS1-Ser909 for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LATS1 |
| Gene ID | 9113 |
| Uniprot ID | LATS1_HUMAN |
| Immunogen | Synthesized phosho peptide around human LATS1/2 (Ser909) |
| Specificity | This antibody detects endogenous levels of Human LATS1/2 (phospho-Ser909) |
Additional Info
| Post Translational Modifications | Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylated by MAP4Ks.in parallel to STK3/MST2 and resulting to its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. |
| Function | Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1. Acts as an activator of the NLRP3 inflammasome by mediating phosphorylation of 'Ser-265' of NLRP3 following NLRP3 palmitoylation, promoting NLRP3 activation by NEK7. |
| Protein Name | Serine/Threonine-Protein Kinase Lats1Large Tumor Suppressor Homolog 1Warts Protein KinaseH-Warts |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleMidbodySpindle Pole BodyLocalizes To The Centrosomes Throughout Interphase But Migrates To The Mitotic ApparatusIncluding Spindle Pole BodiesMitotic SpindleAnd MidbodyDuring Mitosis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Lats1 antibodyAnti-Large Tumor Suppressor Homolog 1 antibodyAnti-Warts Protein Kinase antibodyAnti-H-Warts antibodyAnti-LATS1 antibodyAnti-WARTS antibody |
Information sourced from Uniprot.org