Western blot analysis of lysates from HeLa cells, using phospho-LATS1-S909/LATS2-S872 Rabbit pAb (STJ11101039) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-LATS1/LATS2-S909/S872 antibody (STJ11101039)

⇓ Datasheet ⇓ SDS Citations
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101039
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General Information

Short DescriptionRabbit polyclonal Phospho-LATS1/LATS2-S909/S872 antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolLATS2
LATS1
Gene ID26524
9113
Uniprot IDLATS2_HUMAN
LATS1_HUMAN
Immunogen SequenceAHSLV
SpecificityA synthetic phosphorylated peptide around S909 of human LATS1 (NP_004681.1).

Additional Info

Tissue Specificity Q9NRM7 - Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
O95835 - Expressed in all adult tissues examined except for lung and kidney.
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Lats2 antibody
Anti-Kinase Phosphorylated During Mitosis Protein antibody
Anti-Large Tumor Suppressor Homolog 2 antibody
Anti-Serine/Threonine-Protein Kinase Kpm antibody
Anti-Warts-Like Kinase antibody
Anti-LATS2 antibody
Anti-KPM antibody
Anti-Serine/Threonine-Protein Kinase Lats1 antibody
Anti-Large Tumor Suppressor Homolog 1 antibody
Anti-Warts Protein Kinase antibody
Anti-H-Warts antibody
Anti-LATS1 antibody
Anti-WARTS antibody

Information sourced from Uniprot.org

Citations

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