• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 rabbit monoclonal antibody (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 ug/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 Rabbit monoclonal antibody (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 rabbit monoclonal antibody (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-JunD-S255 Rabbit monoclonal antibody (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-JUND-S255 antibody [S8MR] (STJ11102638)

SKU:
STJ11102638

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-JunD-S255 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUND
Gene ID: 3727
Uniprot ID: JUND_HUMAN
Immunogen: A synthetic phosphorylated peptide around S255 of human JunD (P17535).
Immunogen Sequence: GESPP
Post Translational Modifications Phosphorylated by MAP kinases MAPK8 and MAPK10.phosphorylation is inhibited in the presence of MEN1.
Function Transcription factor binding AP-1 sites. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGAGCTCA-5' and enhancing their transcriptional activity.
Protein Name Transcription Factor Jund
Transcription Factor Ap-1 Subunit Jund
Database Links Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Jund antibody
Anti-Transcription Factor Ap-1 Subunit Jund antibody
Anti-JUND antibody

Information sourced from Uniprot.org

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