• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse liver using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded mouse liver using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded human colon carcinoma using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at dilution of 1:2000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit polyclonal antibody (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10 rabbit polyclonal antibody (STJ11100981) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-Histone H3-S10 antibody (STJ11100981)

SKU:
STJ11100981

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3-S10 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S10 of human Histone H3 (NP_003484.1).
Immunogen Sequence: ARKST
Background This gene encodes a member of the glycosyltransferase 29 family, a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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