• Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-HIRA-Thr555 antibody (521-570 aa) (STJ91346)

SKU:
STJ91346

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein HIRA-Thr555 (521-570 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIRA
Gene ID: 7290
Uniprot ID: HIRA_HUMAN
Immunogen Region: 521-570 aa
Specificity: Phospho-HIRA (T555) Polyclonal Antibody detects endogenous levels of HIRA protein only when phosphorylated at T555.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HIRA around the phosphorylation site of Thr555 at the amino acid range 521-570
Post Translational Modifications Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
Function Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Protein Name Protein Hira
Tup1-Like Enhancer Of Split Protein 1
Database Links Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Pml Body
Primarily
Though Not Exclusively
Localized To The Nucleus
Localizes To Pml Bodies Immediately Prior To Onset Of Senescence
Alternative Antibody Names Anti-Protein Hira antibody
Anti-Tup1-Like Enhancer Of Split Protein 1 antibody
Anti-HIRA antibody
Anti-DGCR1 antibody
Anti-HIR antibody
Anti-TUPLE1 antibody

Information sourced from Uniprot.org

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