| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Forkhead box protein O4-Thr451 (417-466 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FOXO4 |
| Gene ID: |
4303 |
| Uniprot ID: |
FOXO4_HUMAN |
| Immunogen Region: |
417-466 aa |
| Specificity: |
Phospho-FoxO4 (T451) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at T451. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human FOXO4 around the phosphorylation site of Thr451 at the amino acid range 417-466 |
| Post Translational Modifications | Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability. |
| Function | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. |
| Protein Name | Forkhead Box Protein O4Fork Head Domain Transcription Factor Afx1 |
| Database Links | Reactome: R-HSA-198693Reactome: R-HSA-5674400Reactome: R-HSA-5689880Reactome: R-HSA-9614399Reactome: R-HSA-9614657Reactome: R-HSA-9615017Reactome: R-HSA-9617629Reactome: R-HSA-9617828Reactome: R-HSA-9733709 |
| Cellular Localisation | CytoplasmNucleusWhen PhosphorylatedTranslocated From Nucleus To CytoplasmDephosphorylation Triggers Nuclear TranslocationMonoubiquitination Increases Nuclear LocalizationWhen Deubiquitinated |
| Alternative Antibody Names | Anti-Forkhead Box Protein O4 antibodyAnti-Fork Head Domain Transcription Factor Afx1 antibodyAnti-FOXO4 antibodyAnti-AFX antibodyAnti-AFX1 antibodyAnti-MLLT7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
