• Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (STJ22263) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (STJ22263) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-MAPK6-S189 antibody (STJ22263)

SKU:
STJ22263

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Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ERK3-S189 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK6
Gene ID: 5597
Uniprot ID: MK06_HUMAN
Immunogen: A synthetic phosphorylated peptide around S189 of human ERK3 (NP_002739.1).
Immunogen Sequence: HLSEG
Tissue Specificity Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Post Translational Modifications Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
Function Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.
Protein Name Mitogen-Activated Protein Kinase 6
Map Kinase 6
Mapk 6
Extracellular Signal-Regulated Kinase 3
Erk-3
Map Kinase Isoform P97
P97-Mapk
Database Links Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Nucleus
Translocates To The Cytoplasm Following Interaction With Mapkapk5
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 6 antibody
Anti-Map Kinase 6 antibody
Anti-Mapk 6 antibody
Anti-Extracellular Signal-Regulated Kinase 3 antibody
Anti-Erk-3 antibody
Anti-Map Kinase Isoform P97 antibody
Anti-P97-Mapk antibody
Anti-MAPK6 antibody
Anti-ERK3 antibody
Anti-PRKM6 antibody

Information sourced from Uniprot.org

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