| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Epidermal growth factor receptor substrate 15-Tyr849 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPS15 |
| Gene ID: |
2060 |
| Uniprot ID: |
EPS15_HUMAN |
| Specificity: |
Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849. |
| Immunogen: |
Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849) |
| Post Translational Modifications | Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Ubiquitinated. |
| Function | Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. |
| Protein Name | Epidermal Growth Factor Receptor Substrate 15Protein Eps15Protein Af-1p |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-6807004Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideMembraneClathrin-Coated PitRecruited To The Plasma Membrane Upon Egfr Activation And Localizes To Coated PitsColocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments And In Cytoplasmic Juxtanuclear Structures Called AggresomesIsoform 2: Early Endosome MembraneColocalizes With Hgs On Bilayered Clathrin Coats On Endosomes |
| Alternative Antibody Names | Anti-Epidermal Growth Factor Receptor Substrate 15 antibodyAnti-Protein Eps15 antibodyAnti-Protein Af-1p antibodyAnti-EPS15 antibodyAnti-AF1P antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
