• Western blot analysis of extracts of Jurkat cells, using Phospho-eNOS-S1177 antibody (STJ22320). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit polyclonal antibody (A1548). HeLa cells were treated by IGF-1 (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of HeLa cells, using Phospho-NOS3-S1177 polyclonal antibody (STJ22320) at 1:1000 dilution or eNOS antibody (A1548). HeLa cells were treated by IGF-1 (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit polyclonal antibody (STJ22320). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-NOS3-S1177 antibody (STJ22320)

SKU:
STJ22320

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-eNOS-S1177 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOS3
Gene ID: 4846
Uniprot ID: NOS3_HUMAN
Immunogen: A synthetic phosphorylated peptide around S1177 of human eNOS (NP_000594.2).
Immunogen Sequence: TQSFS
Tissue Specificity Platelets, placenta, liver and kidney.
Post Translational Modifications Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Protein Name Nitric Oxide Synthase 3
Constitutive Nos
Cnos
Ec-Nos
Nos Type Iii
Nosiii
Nitric Oxide Synthase - Endothelial
Endothelial Nos
Enos
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1474151
Reactome: R-HSA-203615
Reactome: R-HSA-203641
Reactome: R-HSA-203754
Reactome: R-HSA-392154
Reactome: R-HSA-5218920
Reactome: R-HSA-9009391
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytoskeleton
Golgi Apparatus
Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle
Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative Antibody Names Anti-Nitric Oxide Synthase 3 antibody
Anti-Constitutive Nos antibody
Anti-Cnos antibody
Anti-Ec-Nos antibody
Anti-Nos Type Iii antibody
Anti-Nosiii antibody
Anti-Nitric Oxide Synthase - Endothelial antibody
Anti-Endothelial Nos antibody
Anti-Enos antibody
Anti-NOS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance