• Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~50 kDa eEF1A2 protein phosphorylated at Ser358 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).

Anti-Phospho-EEF1A2-Ser358 antibody (STJA0003624)

SKU:
STJA0003624-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Chicken/Non-Human Primates/Rat/Sheep/Xenopus
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-eEF1A2-Ser358 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1A2
Gene ID: 1917
Uniprot ID: EF1A2_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH.
Tissue Specificity Brain, heart, and skeletal muscle.
Post Translational Modifications Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant.
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protein Name Elongation Factor 1-Alpha 2
Ef-1-Alpha-2
Eukaryotic Elongation Factor 1 A-2
Eef1a-2
Statin-S1
Database Links Reactome: R-HSA-156842
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Elongation Factor 1-Alpha 2 antibody
Anti-Ef-1-Alpha-2 antibody
Anti-Eukaryotic Elongation Factor 1 A-2 antibody
Anti-Eef1a-2 antibody
Anti-Statin-S1 antibody
Anti-EEF1A2 antibody
Anti-EEF1AL antibody
Anti-STN antibody

Information sourced from Uniprot.org

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