• Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-DCX-Ser378 antibody (STJ193223)

SKU:
STJ193223

Current Stock:
Host: Rabbit
Applications: IHC/IF/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Neuronal migration protein doublecortin-Ser378 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCX
Gene ID: 1641
Uniprot ID: DCX_HUMAN
Specificity: This antibody detects endogenous phospho levels of Doublecortin (Phospho-Ser378) at Human:S378, Mouse:S297, Rat:S297
Immunogen: Synthesized peptide derived from the human Doublecortin (Phospho-Ser378)
Post Translational Modifications Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Protein Name Neuronal Migration Protein Doublecortin
Doublin
Lissencephalin-X
Lis-X
Database Links Reactome: R-HSA-447043
Cellular Localisation Cytoplasm
Cell Projection
Neuron Projection
Localizes At Neurite Tips
Alternative Antibody Names Anti-Neuronal Migration Protein Doublecortin antibody
Anti-Doublin antibody
Anti-Lissencephalin-X antibody
Anti-Lis-X antibody
Anti-DCX antibody
Anti-DBCN antibody
Anti-LISX antibody

Information sourced from Uniprot.org

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