Anti-Phospho-DCX-Ser378 antibody (STJ193223)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human Doublecortin (Phospho-Ser378)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Neuronal migration protein doublecortin-Ser378 for use in IHC, IF and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:50-200WB 1:500-2000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DCX |
| Gene ID | 1641 |
| Uniprot ID | DCX_HUMAN |
| Immunogen | Synthesized peptide derived from the human Doublecortin (Phospho-Ser378) |
| Specificity | This antibody detects endogenous phospho levels of Doublecortin (Phospho-Ser378) at Human:S378, Mouse:S297, Rat:S297 |
Additional Info
| Post Translational Modifications | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
| Function | Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. |
| Protein Name | Neuronal Migration Protein DoublecortinDoublinLissencephalin-XLis-X |
| Database Links | Reactome: R-HSA-447043 |
| Cellular Localisation | CytoplasmCell ProjectionNeuron ProjectionLocalizes At Neurite Tips |
| Alternative Antibody Names | Anti-Neuronal Migration Protein Doublecortin antibodyAnti-Doublin antibodyAnti-Lissencephalin-X antibodyAnti-Lis-X antibodyAnti-DCX antibodyAnti-DBCN antibodyAnti-LISX antibody |
Information sourced from Uniprot.org