• Western blot analysis of lysates from 293 cells, using Cortactin (Phospho-Tyr466) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500
  • Western blot analysis of 293 cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500
  • Immunofluorescence analysis of Hela cell. 1, Cortactin (phospho Tyr466) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-Cortactin (Y466) antibody (STJ91006)

SKU:
STJ91006

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat, Monkey
Short Description: Rabbit polyclonal anti-Phospho-Cortactin (Y466) antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity: Phospho-Cortactin (Y466) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y466.
Uniprot ID: SRC8_HUMAN
Gene ID: 2017
Gene Symbol: CTTN
Immunogen: Synthesized peptide derived from human Cortactin around the phosphorylation site of Y466.
Immunogen Region: 410-490 aa
Function Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane.
Protein Name Src Substrate Cortactin
Amplaxin
Oncogene Ems1
Database Links Reactome: R-HSA-5627123
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Ruffle
Dendrite
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Podosome
Cell Junction
Focal Adhesion
Membrane
Clathrin-Coated Pit
Dendritic Spine
Cell Cortex
Colocalizes Transiently With Ptk2/Fak1 At Focal Adhesions
Associated With Membrane Ruffles And Lamellipodia
In The Presence Of Cttnbp2nl
Colocalizes With Stress Fibers
In The Presence Of Cttnbp2
Localizes At The Cell Cortex
In Response To Neuronal Activation By Glutamate
Redistributes From Dendritic Spines To The Dendritic Shaft
Colocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones
Alternative Antibody Names Anti-Src Substrate Cortactin antibody
Anti-Amplaxin antibody
Anti-Oncogene Ems1 antibody
Anti-CTTN antibody
Anti-EMS1 antibody

Information sourced from Uniprot.org

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