• Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunohistochemistry analysis of paraffin-embedded human brain, using Claudin 5 (Phospho-Tyr217) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN5-Tyr217 antibody (169-218 aa) (STJ91249)

SKU:
STJ91249

Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Claudin-5-Tyr217 (169-218 aa) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN5
Gene ID: 7122
Uniprot ID: CLD5_HUMAN
Immunogen Region: 169-218 aa
Specificity: Phospho-Claudin-5 (Y217) Polyclonal Antibody detects endogenous levels of Claudin-5 protein only when phosphorylated at Y217.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Claudin 5 around the phosphorylation site of Tyr217 at the amino acid range 169-218
Protein Name Claudin-5
Transmembrane Protein Deleted In Vcfs
Tmdvcf
Database Links Reactome: R-HSA-420029
Reactome: R-HSA-8935964
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Claudin-5 antibody
Anti-Transmembrane Protein Deleted In Vcfs antibody
Anti-Tmdvcf antibody
Anti-CLDN5 antibody
Anti-AWAL antibody
Anti-TMVCF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance