Anti-Phospho-CDC25B-Ser323 antibody (289-338 aa) (STJ90217)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDC25B around the phosphorylation site of Ser323 at the amino acid range 289-338
General Information
| Short Description | Rabbit polyclonal anti-Phospho-M-phase inducer phosphatase 2-Ser323 (289-338 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC25B |
| Gene ID | 994 |
| Uniprot ID | MPIP2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CDC25B around the phosphorylation site of Ser323 at the amino acid range 289-338 |
| Immunogen Region | 289-338 aa |
| Specificity | Phospho-Cdc25B (S323) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S323. |
Additional Info
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity. |
| Protein Name | M-Phase Inducer Phosphatase 2Dual Specificity Phosphatase Cdc25b |
| Database Links | Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-8862803Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole |
| Alternative Antibody Names | Anti-M-Phase Inducer Phosphatase 2 antibodyAnti-Dual Specificity Phosphatase Cdc25b antibodyAnti-CDC25B antibodyAnti-CDC25HU2 antibody |
Information sourced from Uniprot.org