Anti-Phospho-CDC16-Ser560 antibody (526-575 aa) (STJ91091)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDC16/APC6 around the phosphorylation site of Ser560 at the amino acid range 526-575
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Cell division cycle protein 16 homolog-Ser560 (526-575 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC16 |
| Gene ID | 8881 |
| Uniprot ID | CDC16_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CDC16/APC6 around the phosphorylation site of Ser560 at the amino acid range 526-575 |
| Immunogen Region | 526-575 aa |
| Specificity | Phospho-Cdc16 (S560) Polyclonal Antibody detects endogenous levels of Cdc16 protein only when phosphorylated at S560. |
Additional Info
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. |
| Protein Name | Cell Division Cycle Protein 16 HomologAnaphase-Promoting Complex Subunit 6Apc6Cdc16 HomologCdc16hsCyclosome Subunit 6 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindleColocalizes With Cdc27 To The Centrosome At All Stages Of The Cell Cycle And To The Mitotic Spindle |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 16 Homolog antibodyAnti-Anaphase-Promoting Complex Subunit 6 antibodyAnti-Apc6 antibodyAnti-Cdc16 Homolog antibodyAnti-Cdc16hs antibodyAnti-Cyclosome Subunit 6 antibodyAnti-CDC16 antibodyAnti-ANAPC6 antibody |
Information sourced from Uniprot.org