Host: | Rabbit |
Applications: | IHC/WB |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-Phospho-G1/S-specific cyclin-E1-Ser399 is suitable for use in Immunohistochemistry and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-2000IHC 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CCNE1 |
Gene ID: | 898 |
Uniprot ID: | CCNE1_HUMAN |
Specificity: | This antibody detects endogenous levels of Cyclin E1 (Phospho Ser399) Rabbit pAb at Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from human Cyclin E1 (Phospho Ser399) |
Tissue Specificity | Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. |
Post Translational Modifications | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation. |
Function | Essential for the control of the cell cycle at the G1/S (start) transition. |
Protein Name | G1/S-Specific Cyclin-E1 |
Database Links | Reactome: R-HSA-1538133Reactome: R-HSA-187577Reactome: R-HSA-2559586Reactome: R-HSA-390471Reactome: R-HSA-6804116Reactome: R-HSA-69017Reactome: R-HSA-69200Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-8849470Reactome: R-HSA-9661069Reactome: R-HSA-9706019 |
Cellular Localisation | Nucleus |
Alternative Antibody Names | Anti-G1/S-Specific Cyclin-E1 antibodyAnti-CCNE1 antibodyAnti-CCNE antibody |
Information sourced from Uniprot.org