• Western Blot analysis of HeLa mouse heart using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-CCNE1-Ser399 antibody (STJA0010041)

SKU:
STJA0010041

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-G1/S-specific cyclin-E1-Ser399 is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE1
Gene ID: 898
Uniprot ID: CCNE1_HUMAN
Specificity: This antibody detects endogenous levels of Cyclin E1 (Phospho Ser399) Rabbit pAb at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human Cyclin E1 (Phospho Ser399)
Tissue Specificity Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Post Translational Modifications Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation.
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Protein Name G1/S-Specific Cyclin-E1
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-8849470
Reactome: R-HSA-9661069
Reactome: R-HSA-9706019
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E1 antibody
Anti-CCNE1 antibody
Anti-CCNE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance