| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-E3 ubiquitin-protein ligase CBL-Tyr674 (640-689 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBL |
| Gene ID: |
867 |
| Uniprot ID: |
CBL_HUMAN |
| Immunogen Region: |
640-689 aa |
| Specificity: |
Phospho-Cbl (Y674) Polyclonal Antibody detects endogenous levels of Cbl protein only when phosphorylated at Y674. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human CBL around the phosphorylation site of Tyr674 at the amino acid range 640-689 |
| Function | Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Ubiquitinates SPRY2. Ubiquitinates EGFR. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA. |
| Protein Name | E3 Ubiquitin-Protein Ligase CblCasitas B-Lineage Lymphoma Proto-OncogeneProto-Oncogene C-CblRing Finger Protein 55Ring-Type E3 Ubiquitin Transferase CblSignal Transduction Protein Cbl |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-1236382Reactome: R-HSA-1295596Reactome: R-HSA-1433559Reactome: R-HSA-182971Reactome: R-HSA-2173789Reactome: R-HSA-5637810Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-6807004Reactome: R-HSA-8849469Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8875360Reactome: R-HSA-912631Reactome: R-HSA-9680350Reactome: R-HSA-9706369Reactome: R-HSA-9706377 |
| Cellular Localisation | CytoplasmCell MembraneCell ProjectionCiliumGolgi ApparatusColocalizes With Fgfr2 In Lipid Rafts At The Cell Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Cbl antibodyAnti-Casitas B-Lineage Lymphoma Proto-Oncogene antibodyAnti-Proto-Oncogene C-Cbl antibodyAnti-Ring Finger Protein 55 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Cbl antibodyAnti-Signal Transduction Protein Cbl antibodyAnti-CBL antibodyAnti-CBL2 antibodyAnti-RNF55 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
