| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase BTK-Tyr223 (188-237 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BTK |
| Gene ID: |
695 |
| Uniprot ID: |
BTK_HUMAN |
| Immunogen Region: |
188-237 aa |
| Specificity: |
Phospho-Btk (Y223) Polyclonal Antibody detects endogenous levels of Btk protein only when phosphorylated at Y223. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human BTK around the phosphorylation site of Tyr223 at the amino acid range 188-237 |
| Post Translational Modifications | Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment. |
| Function | Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK also plays a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis. |
| Protein Name | Tyrosine-Protein Kinase BtkAgammaglobulinemia Tyrosine KinaseAtkB-Cell Progenitor KinaseBpkBruton Tyrosine Kinase |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-2029482Reactome: R-HSA-2424491Reactome: R-HSA-2871809Reactome: R-HSA-416476Reactome: R-HSA-416482Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5663213Reactome: R-HSA-8964315Reactome: R-HSA-9664422Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinNucleusMembrane RaftIn Steady StateBtk Is Predominantly CytosolicFollowing B-Cell Receptor (Bcr) Engagement By AntigenTranslocates To The Plasma Membrane Through Its Ph DomainPlasma Membrane Localization Is A Critical Step In The Activation Of BtkA Fraction Of Btk Also Shuttles Between The Nucleus And The CytoplasmAnd Nuclear Export Is Mediated By The Nuclear Export Receptor Crm1 |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Btk antibodyAnti-Agammaglobulinemia Tyrosine Kinase antibodyAnti-Atk antibodyAnti-B-Cell Progenitor Kinase antibodyAnti-Bpk antibodyAnti-Bruton Tyrosine Kinase antibodyAnti-BTK antibodyAnti-AGMX1 antibodyAnti-ATK antibodyAnti-BPK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
