STJ70913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma mamebrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PGRMC2 antibody (C-Term) (STJ70913)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70913-100
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General Information

Short DescriptionGoat polyclonal antibody anti-PGRMC2 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Pig/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:1000.
IF: Strong expression of the protein seen in the cytoplasm and plasma membranes of HeLa cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of HeLa cells. Recommended c

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolPGRMC2
Gene ID10424
Uniprot IDPGRC2_HUMAN
Accession NumberNP_006311.3
Immunogen RegionC-Term
Immunogen SequenceDEEDTKDHNKQD

Additional Info

Function Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. May serve as a universal non-classical progesterone receptor in the uterus (Probable). Intracellular heme chaperone required for delivery of labile, or signaling heme, to the nucleus. Plays a role in adipocyte function and systemic glucose homeostasis. In brown fat, which has a high demand for heme, delivery of labile heme in the nucleus regulates the activity of heme-responsive transcriptional repressors such as NR1D1 and BACH1.
Protein Name Membrane-Associated Progesterone Receptor Component 2
Progesterone Membrane-Binding Protein
Steroid Receptor Protein Dg6
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-9707616
Cellular Localisation Membrane
Single-Pass Membrane Protein
Nucleus Envelope
Endoplasmic Reticulum
Secreted
Alternative Antibody Names Anti-Membrane-Associated Progesterone Receptor Component 2 antibody
Anti-Progesterone Membrane-Binding Protein antibody
Anti-Steroid Receptor Protein Dg6 antibody
Anti-PGRMC2 antibody
Anti-DG6 antibody
Anti-PMBP antibody

Information sourced from Uniprot.org

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